RevoluGen’s HMW Fireplace Monkey Automated DNA Extraction Validated on Tecan Robotic Helps Fast Progress in Multiplexed DNA Sequencing
LONDON–(BUSINESS WIRE) – UK genomic instruments developer RevoluGen Ltd. (RevoluGen or the corporate), at this time proclaims the technological validation of the world’s first automated extraction of library-ready excessive molecular weight DNA (HMW) in a multi-well filter plate format.
As a part of its collaboration settlement with Tecan, introduced in 2020, RevoluGen has accomplished configuration and validation assessments (by DNA sequencing) of a bespoke and absolutely automated workflow that comes with a Tecan Fluent robotic liquid manipulator 780 matched with an built-in Rotanda 460 robotic centrifuge utilizing laboratory commonplace multi-well plates, with patented Fireplace Monkey chemistry and matrix, permitting automation of the spin-based course of.
The brand new Fireplace Monkey HMW-DNA automated extraction protocol has been validated to extract and purify bacterial samples. Optimization of multi-well filter plates is underway in RevoluGen’s R&D laboratories with the business launch of the Fireplace Monkey HMW-DNA automated extraction package anticipated within the coming months.
“The automation of DNA extraction by RevoluGen, the important first step within the DNA sequencing course of, is well timed as a result of monitoring for structural variants might result in a sequencing growth,” says the CEO of the corporate, Pieter Haitsma Mulier. A current report in a number one commerce publication BioCentury concluded that the push to maintain COVID-19 variants at bay may very well be the purpose that turns genomic sequencing from a complicated approach right into a extensively accessible genomic device, with main corporations targeted on automation.
Within the validation cycle to display the standard of the sequencing output, eight E. coli the cell suspensions have been transferred to a 96-well plate and, after a completely handbook course of, the output DNA supplied sequencing outcomes equal to the manually extracted Fireplace Monkey spin column-based HMW-column DNA. The common strand size obtained was 106 kb with sequencing on the Oxford Nanopore (ONT) MinION LSK110 platform delivering over 30 GB at N50 over 30 kb, with good pore well being (Determine 1).
RevoluGen Fireplace Monkey / Fireplace Flower Nucleic Acid Isolation and Purification (NAIP) expertise relies on a spin-based protocol to extract HMW DNA utilizing excessive g power that doesn’t break down d molecules. ‘DNA lengthy and fragile. It produces DNA that accommodates DNA fragments which might be neither too quick nor too lengthy, as this improves the general sequencing course of by not losing sequencing sources by studying the smallest, much less helpful fragments or killing components of the studying expertise by blocking the pores ONT with fragments. which might be too lengthy.
“The extraction of HMW DNA based mostly on an ordinary laboratory rotary column methodology was revolutionary in itself. Given the demand for automation, we labored with Tecan to understand a purposeful robotic system that absolutely automates the method of extracting DNA from lengthy size DNA utilizing a multi-well plate course of. tailored. This can be a actually disruptive innovation that may assist massively excessive throughput, top quality and price efficient DNA sequencing, ”says Dr Georgios Patsos, inventor of Fireplace Monkey and CSO expertise at RevoluGen.
The fast progress of sequencing world wide will clearly require automated NAIP and sequencing processes to handle the amount of samples wanted for purposes corresponding to inhabitants genomics, screening for epidemiological mutations, and monitoring of microbial resistance genes. antibiotics.
Dr Gemma Langridge, Group Chief on the Quadram Institute within the UK, who was one of many early adopters of RevoluGen’s Fireplace Monkey expertise, added: “With automation coming from RevoluGen, what we’re on the lookout for, it’s to have the ability to put together tons of of micro organism. strains at a time, then the scope of our analysis will increase dramatically on this means.
Persevering with, “We look ahead to persevering with our relationship with RevoluGen to discover the position of their expertise in metagenomic evaluation and to discover automation.”
Whereas the COVID-19 pandemic has highlighted the advantages of DNA sequencing-based viral genomic epidemiology, international surveillance can also be important to trace antibiotic resistance genes to sort out the issue. World Microbial Resistance to Antibiotics (AMR). For instance, the Quadram groups have revealed constructive outcomes utilizing Fireplace Monkey with Illumina Entire-Genome Sequencing to determine drug resistance (XDR). Salmonella typhi in addition to research to determine mutations linked to ciprofloxacin heteroresistance, i.e. a inhabitants of micro organism with completely different sensitivity to an antibiotic utilizing Fireplace Monkey to extract DNA from Salmonella enterica remoted hen meat.
Different high-volume DNA sequencing purposes embrace inhabitants genomics research that the majority nations are starting to undertake and an rising strategy to genomic screening of most cancers sufferers for extra correct prognosis and administration of therapies. These purposes all have in frequent the necessity for automated processes able to dealing with the massive variety of affected person samples that will probably be required.
At present, every spherical of sequencing requires that every DNA pattern be extracted individually. A validated and automatic Fireplace Monkey course of for automated multiplexed DNA-HMW extraction will additional scale back the general price of sequencing and thereby speed up sequencing adoption in lots of excessive quantity dependent purposes. The DNA sequencing market is at the moment projected to develop from $ 4 billion to roughly $ 50 billion over the following 10 years.
“Automation is a revolutionary differentiator and can enable us to quickly advance sequencing at a decrease unit price. Now that we’ve validated the automated protocol, we imagine Fireplace Monkey is effectively on its option to turning into the DNA extraction expertise of alternative for top quantity purposes. Our expertise combines quick learn and lengthy learn sequencing, permitting each from the identical pattern. It affords the flexibleness to overview the identical authentic pattern for extra detailed long-read sequencing after a primary run with cheaper short-read sequencing, ”concludes RevoluGen CEO Pieter Haitsma Mulier.
About RevoluGen – www.revolugen.co.uk.
RevoluGen is a personal scientific analysis and growth firm that commercializes molecular instruments with a specific concentrate on the fast extraction of lengthy and pure DNA fragments from cells.
RevoluGen’s Nucleic Acid Isolation and Purification (NAIP) merchandise have vast utility briefly and lengthy learn DNA sequencing, together with inhabitants genomics, screening for epidemiological mutations, and gene monitoring. microbial resistance to antibiotics.
Fireplace Monkey dramatically improves sequencing outcomes by extracting lengthy fragment DNA with a simultaneous dimension choice function constructed into the protocol that minimizes contamination of small fragment DNA. Used independently, the Fireplace Flower dimension choice protocol can enhance the molecular ratio of any extraction package and is appropriate with all sequencing applied sciences.
Firm headquarters, R&D, manufacturing and direct buyer gross sales and assist are based mostly within the UK. RevoluGen serves prospects worldwide and has agreements with main international molecular biology device corporations together with Merck KGaA (gross sales), Cytiva (manufacturing), Tecan (automation) and A4P (logistics).